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Jackson|二抗709-005-149*
通過免疫親和層析從抗血清分離全IgG抗體作為完整分子。 它們具有Fc部分和兩個通過二硫鍵連接在一起的抗原結合Fab部分,因此它們是二價的。 據報道平均分子量約為160kDa。 整個IgG形式的抗體適用于大多數免疫檢測程序,并且是成本效益的。
基于免疫電泳和/或ELISA,抗體與全分子人IgG反應。 它還與其他人免疫球蛋白的輕鏈反應。 未檢測到針對非免疫球蛋白血清蛋白的抗體。 已經通過ELISA和/或固相吸附測試抗體以確保與牛,雞,山羊,豚鼠,敘利亞倉鼠,馬,小鼠,兔,大鼠和綿羊血清蛋白的小交叉反應,但它可以交叉 - 與來自其他物種的免疫球蛋白反應。
Jackson|二抗709-005-149*
Physical State: Sterile-filtered liquid
Storage:
Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.
Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: None
Suggested Working Concentration or Dilution Range:
10-20 µg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
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